Biotium Introduces New Highly-Selective Stains For Both Gram-Positive and Gram-Negative Bacteria

26 October 2023 | Thursday | News


New BactoView™ Dead Stains and BactoView™ Viability Kits help simplify and improve highly selective dead cell staining
Live or heat-killed cultures of B. subtilis stained with the BactoView™ Viability Green/Red kit and imaged by confocal microscopy. Dead cells are stained red with BactoView™ Dead 570/585, all cells are stained green with BactoView™ Green Viability Counterstain. Co-stained cells appear yellow in the merged images.

Live or heat-killed cultures of B. subtilis stained with the BactoView™ Viability Green/Red kit and imaged by confocal microscopy. Dead cells are stained red with BactoView™ Dead 570/585, all cells are stained green with BactoView™ Green Viability Counterstain. Co-stained cells appear yellow in the merged images.

Biotium, a leading innovator of fluorescent dyes for life science research, proudly announces the launch of its breakthrough BactoView™ Dead Stains and BactoView™ Viability Kits. These products were carefully designed for easy usage and improved dead cell selectivity for enhanced microbial viability determination. With precise dead cell detection being vital for accurate analysis, Biotium’s BactoView™ Dead Stains deliver significant improvements in this procedure.

Unlike the traditional viability dyes propidium iodide and ethidium homodimer, Biotium’s BactoView™ Dead Stains provide brighter dead cell staining with minimal background interference. The specially-designed chemical structures of the dyes effectively exclude them from live cells of both gram-negative and particularly gram-positive strains, selectively labeling dead cells. There is only a single 30-minute staining step with no additional wash requirements for ease-of-use, with the flexibility of seven available dye colors ranging from green to near-IR for microscopy or flow cytometry analysis.
 
“These dyes and kits are a much-needed improvement for microbiological research, particularly for gram-positive stains that don’t work well with existing dead cell dyes,” Biotium’s Director of Bioscience, Dr. Lori Roberts, excitedly comments. With both BactoView™ Dead Stains and Viability Kits, researchers can now attain unprecedented flexibility and accuracy in their experiments. These products grow Biotium's vast repertoire as part of our commitment to life science innovation.

 

 

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