Bioprocess scale-up poses challenges in maintaining growth kinetics, productivity, cell health, and mass transfer. Effective control of parameters like agitation, dissolved O2 and CO2, and nutrient supplementation is crucial. The Thermo Scientific™ DynaDrive™ Single-Use Bioreactor (S.U.B.) addresses these challenges with advanced technologies, enhancing mass transfer and gas control. An example demonstrates successful scaling of a fed-batch process from 50 L to 500 L, using both the Thermo Scientific™ 5:1 HyPerforma™ S.U.B. and DynaDrive S.U.B. product lines, achieving consistent growth and product yield despite varying gassing and agitation controls.