The application of Difco™ TC Yeastolate Ultra-Filtered (TCY UF) in cell culture supplements led to over a 100% increase in titer yield, significantly optimizing productivity for bioreactor processes. This innovative approach replaced a chemically defined CHO medium with a higher-performing medium and feed pairing strategy. The key findings highlight the efficacy of TCY UF in improving bioreactor scalability and productivity.
Over 100% Titer Improvement: Utilizing TCY UF in feed strategies resulted in more than double the antibody yield compared to traditional feeds.
Optimized Feed Strategy: A targeted feed approach, including 3 g/L of TCY UF fed at day 5, increased titer by approximately 70% over the control.
Enhanced Medium Performance: Combining Medium 1 with TCY UF supplementation achieved over a 50% improvement in titer yield.
Scalable Results: The optimized process was successfully scaled from shake flask studies to bench-scale bioreactors, maintaining high productivity levels.